Cloning of SV40 genomes from human brain tumors

Virology. 1984 Oct 30;138(2):336-40. doi: 10.1016/0042-6822(84)90357-x.

Abstract

From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early region removing most of the large T-coding region. In addition, this mutant also carries a tandem duplication of an intact origin of replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Astrocytoma / microbiology*
  • Brain Neoplasms / microbiology*
  • Chromosome Deletion
  • Cloning, Molecular
  • DNA Replication
  • DNA Restriction Enzymes
  • DNA, Viral / genetics
  • Gene Expression Regulation
  • Humans
  • Meningioma / microbiology*
  • Simian virus 40 / genetics*
  • Virus Replication

Substances

  • DNA, Viral
  • DNA Restriction Enzymes