In Vitro and in Vivo Transcription Initiation Sites on the TK-encoding BamHI Q Fragment of HSV-1 DNA

Virology. 1984 Oct 30;138(2):368-72. doi: 10.1016/0042-6822(84)90363-5.

Abstract

The sites of in vitro transcription initiation on the BamHI Q fragment of herpes simplex virus DNA have been compared with the sites of 5' ends of RNAs made in vivo after virus infection. S1-nuclease protection analysis of these RNAs shows that there are in vivo counterparts for each of the five previously identified in vitro transcripts. The whole-cell-extract RNA polymerase II transcription system faithfully initiates RNAs predominantly at bona fide in vivo start sites and gives few, if any, false positive start sites. Further, antiparallel, self-complementary transcripts from the BamHI Q fragment were observed in the coding region of the HSV thymidine kinase gene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • Endonucleases
  • Genes, Viral
  • Molecular Weight
  • RNA Polymerase II / genetics
  • RNA, Messenger / genetics
  • RNA, Viral / genetics
  • Simplexvirus / genetics*
  • Single-Strand Specific DNA and RNA Endonucleases
  • Thymidine Kinase / genetics
  • Transcription, Genetic*

Substances

  • RNA, Messenger
  • RNA, Viral
  • Thymidine Kinase
  • RNA Polymerase II
  • Endonucleases
  • Single-Strand Specific DNA and RNA Endonucleases