Studies of peripheral blood monocytes in pulmonary sarcoidosis

Clin Exp Immunol. 1984 Nov;58(2):357-63.

Abstract

In 14 patients with pulmonary sarcoidosis and 14 matched controls we studied peripheral blood lymphocyte and monocyte counts, distribution of T and B lymphocytes, the functional helper:suppressor T cell balance, the antibody-dependent cell-mediated cytotoxicity of monocytes (monocyte ADCC), and the capacity of peripheral monocytes to generate angiotensin converting enzyme (ACE) in culture. Apart from lymphopenia in sarcoidosis patients we found a normal lymphocyte subset distribution and no evidence of increased suppressor T cell activity, using a PWM driven proliferative assay. The patients exhibited a normal monocyte count, but the proportion of monocytes was increased in sarcoidosis. Patients with active sarcoidosis had a significantly increased monocyte ADCC which was positively correlated with raised serum ACE. Peripheral monocytes had a measurable, but low ACE activity, which was modestly higher in active sarcoidosis than in controls. We could not reproduce earlier reported results on a glucocorticoid induced ACE synthesis from cultured human monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibody-Dependent Cell Cytotoxicity
  • Cells, Cultured
  • Female
  • Humans
  • Leukocyte Count
  • Lung Diseases / blood*
  • Lung Diseases / immunology
  • Male
  • Middle Aged
  • Monocytes / enzymology
  • Monocytes / immunology*
  • Peptidyl-Dipeptidase A / metabolism
  • Sarcoidosis / blood*
  • Sarcoidosis / immunology
  • T-Lymphocytes, Helper-Inducer / immunology
  • T-Lymphocytes, Regulatory / immunology

Substances

  • Peptidyl-Dipeptidase A