Isolation and bacterial expression of a murine alpha leukocyte interferon gene

J Interferon Res. Fall 1984;4(4):635-43. doi: 10.1089/jir.1984.4.635.

Abstract

A murine alpha interferon gene (MuIFN-alpha A) was isolated from a murine genomic DNA library in phage lambda. The gene was cloned and its sequence determined. It was expressed in Escherichia coli under the control of the trp promoter/operator which resulted in antiviral activity on mouse L-cells. MuIFN-alpha A exhibits 60% homology to HuIFN-alpha A at the amino acid level.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Gene Expression Regulation
  • Genes
  • Genetic Vectors
  • Humans
  • Interferon Type I / genetics*
  • Mice
  • Operon

Substances

  • Interferon Type I