Plasma beta-endorphin (beta-EP) levels were measured at different stages of metamorphosis in anuran species, namely Rana catesbeiana, R. pipiens, and Xenopus laevis. The mean levels of beta-EP in tadpoles and postmetamorphic juveniles were significantly higher than adult levels in all three species examined. The pattern of high tadpole levels and low adult levels corresponds with the limb regeneration ability of the tadpole and restriction in the adult. We have also reported plasma beta-EP levels in two urodele species, Ambystoma tigrinum and A. jeffersonianum. Localization of beta-EP by immunocytochemistry shows bright fluorescence to beta-EP antiserum in the wound epithelium of the regeneration blastema. The mesoderm and nuclei of all blastema cells remain nonreactive. It is suggested that receptors to beta-EP reside in the epidermis.