The DNA sequence of the BamHI fragments B and the contiguous portion of G extending from the BamHI site to the EcoRI site of the B95-8 strain of Epstein-Barr virus has been determined. The sequence has been analysed for possible protein coding regions and transcriptional control sites. At least eight large open reading frames were found. Several RNA polymerase II promoters have been identified in the BamHI-B fragments. RNAs transcribed from these promoters are substantially induced by treatment of B95-8 cells with 12-O-tetradecanoylphorbol-13-acetate. Phosphonoacetic acid reversed the stimulatory effect of 12-O-tetradecanoylphorbol-13-acetate on the transcription of these RNAs indicating that they are late RNAs.