Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma

Cell. 1984 Dec;39(3 Pt 2):653-62. doi: 10.1016/0092-8674(84)90472-0.


Two distinct nonoverlapping enhancer elements can be defined within the polyoma enhancer region. In mouse fibroblasts, element A provides a 3-fold higher enhancement of the alpha 2-collagen promoter than element B. In mouse embryonal carcinoma cells, element B shows the same efficiency as in fibroblasts, whereas that of element A decreases by a factor of 3.5. Moreover, a single point mutation (PyEC F9.1) increases the efficiency of element B in both cell types, making it superior to element A in embryonal carcinoma cells. The core of element A is located within a 35 bp region tandem duplicated in several wild-type strains, mostly homologous to a crucial repeated sequence of adenovirus E1a enhancer (Hearing and Shenk, 1983). Element B contains the consensus sequence of Weiher et al. (1982). These two homologies are precisely adjacent to the two DNAase l-hypersensitive sites present in viral chromatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cells, Cultured
  • DNA Restriction Enzymes
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation*
  • Genes
  • Genes, Regulator*
  • Genes, Viral
  • Mice
  • Mice, Inbred Strains
  • Plasmids
  • Polyomavirus / genetics*
  • Simian virus 40 / genetics
  • Teratoma
  • Transcription, Genetic
  • Transfection
  • beta-Galactosidase / genetics


  • DNA Restriction Enzymes
  • beta-Galactosidase