A two-dimensional NMR study of the solution structure of a DNA dodecamer comprising the concensus sequence for the specific DNA-binding sites of the glucocorticoid receptor protein

Eur J Biochem. 1984 Dec 17;145(3):629-36. doi: 10.1111/j.1432-1033.1984.tb08603.x.


A two-dimensional 500-MHz 1H-NMR study on the non-self-complementary double-stranded DNA dodecamer 5'd(C-C-A-G-A-A-C-A-G-T-G-G)5'd(C-C-A-C-T-G-T-T-C-T-G-G), is presented. This oligonucleotide contains the consensus octanucleotide sequence 5'd(A-G-A-A-C-A-G-T) for the specific DNA-binding sites of the glucocorticoid receptor protein [Payvar, F. et al. (1984) Cell 35, 381-392]. Using a combination of two-dimensional pure phase absorption nuclear Overhauser enhancement (NOESY) and homonuclear J-correlated (COSY) spectroscopy all non-exchangeable base (with the exception fo the adenine H2 protons), methyl and deoxyribose H1', H2', H2", H3' and H4' resonances are assigned unambiguously using a sequential resonance assignment strategy. From the relative intensities of the cross peaks in the pure phase absorption NOESY spectra at two mixing times it is shown that the dodecamer adopts a B-type conformation in solution.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chemical Phenomena
  • Chemistry
  • Deoxyribonucleotides
  • Magnetic Resonance Spectroscopy
  • Receptors, Cell Surface*
  • Receptors, Glucocorticoid*
  • Receptors, Steroid*
  • Solutions


  • DNA receptor
  • Deoxyribonucleotides
  • Receptors, Cell Surface
  • Receptors, Glucocorticoid
  • Receptors, Steroid
  • Solutions