Cloning of genomic DNA for human atrial natriuretic factor

Biochem Biophys Res Commun. 1984 Dec 14;125(2):797-802. doi: 10.1016/0006-291x(84)90609-0.

Abstract

A human genomic DNA clone for atrial natriuretic factor (ANF) gene was isolated from a human gene library using the previously cloned cDNA for rat ANF as a probe. Partial nucleotide sequencing of the cloned DNA revealed the location of a 120-bp long intron between Lys-41 and Asn-42 of the ANF precursor. Restriction mapping also suggested the existence of at least one other intron in or proximal to the 3' -untranslated region.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Atrial Natriuretic Factor
  • Bacteriophage lambda / metabolism
  • Base Sequence
  • Cloning, Molecular*
  • DNA / isolation & purification*
  • DNA Restriction Enzymes
  • Female
  • Fetus
  • Genes*
  • Humans
  • Liver / metabolism
  • Muscle Proteins / genetics*
  • Nucleic Acid Hybridization
  • Pregnancy
  • Rats
  • Species Specificity

Substances

  • Muscle Proteins
  • NPPA protein, human
  • Atrial Natriuretic Factor
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/K02399