Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12

Mol Gen Genet. 1984;198(2):1-6. doi: 10.1007/BF00328692.


A 37 kb fragment of DNA from an F-prime factor, F100-12, which showed a gene dosage effect on b-type cytochromes, was cloned with a cosmid vector, pHC79. Gel filtration of cytochromes and product analysis of the hybrid plasmids indicated that this fragment contained cybB, the structural gene for cytochrome b561. A chromosomal DNA fragment carrying the cybB gene was cloned by the plaque hybridization technique with Charon 4A as a vector. The gene was subcloned into pBR322 and was located in a 1.3 kb DNA fragment. It was concluded that the cybB gene is located on the chromosome of Escherichia coli K12.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • Cytochrome b Group / genetics*
  • Escherichia coli / genetics*
  • Genes
  • Genes, Bacterial*
  • Genetic Vectors
  • Plasmids


  • Cytochrome b Group
  • cytochrome b561