[Synthesis of a full-length DNA copy of the hemagglutinin gene of the the influenza virus A H1N1 subtype, its cloning and primary structure]

Bioorg Khim. 1984 Nov;10(11):1535-43.
[Article in Russian]


Full-length DNA-copy of hemagglutinin gene RNA of influenza A virus strain A/Leningrad/54/1 was synthesized and cloned in E. coli plasmid pBR 327. Its primary structure was determined by a modified Maxam - Gilbert procedure. The nucleotide sequence of this gene was compared with sequences of analogous genes of influenza strains A/USSR/90/77 and A/PR/8/34. An addition of one nucleotide in non-translated region was found in the former.

Publication types

  • English Abstract

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA / genetics
  • DNA Restriction Enzymes
  • DNA, Viral / biosynthesis*
  • DNA, Viral / genetics
  • Genes, Viral*
  • Hemagglutinins / genetics*
  • Influenza A Virus, H1N1 Subtype*
  • Influenza A virus / genetics*
  • Influenza A virus / immunology
  • Plasmids
  • Templates, Genetic*


  • DNA, Viral
  • Hemagglutinins
  • DNA
  • DNA Restriction Enzymes