Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells

Mol Cell Biol. 1984 Dec;4(12):2929-31. doi: 10.1128/mcb.4.12.2929.

Abstract

The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • DNA Restriction Enzymes / metabolism
  • Drug Resistance
  • Escherichia coli / genetics*
  • Hygromycin B / pharmacology
  • Kanamycin Kinase
  • Moloney murine sarcoma virus / genetics*
  • Phosphotransferases / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Sarcoma Viruses, Murine / genetics*
  • Transfection*

Substances

  • Hygromycin B
  • Phosphotransferases
  • Kanamycin Kinase
  • DNA Restriction Enzymes