Analysis of the ptsH-ptsI-crr region in Escherichia coli K-12: evidence for the existence of a single transcriptional unit

Gene. 1984 Dec;32(1-2):31-40. doi: 10.1016/0378-1119(84)90029-5.

Abstract

A cosmid containing the ptsH, ptsI and crr genes of Escherichia coli coding for three components of the phosphoenolpyruvate: sugar phosphotransferase system (PTS) has been isolated. The products of these genes were identified using the maxicell technique. The cloning of the PTS region as a whole allowed us to map and order ptsH, ptsI and crr genes in a 3.8-kb DNA fragment and determine that ptsI and crr are transcribed from a common promoter. They, therefore, constitute a single transcriptional unit which is likely to include also the ptsH gene. In addition, the crr gene may have a secondary promoter located inside ptsI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Gene Expression Regulation
  • Genes, Bacterial*
  • Genetic Vectors
  • Operon
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
  • Plasmids
  • Transcription, Genetic

Substances

  • DNA, Bacterial
  • Phosphoenolpyruvate Sugar Phosphotransferase System