New Tn10 Derivatives for Transposon Mutagenesis and for Construction of lacZ Operon Fusions by Transposition

Gene. 1984 Dec;32(3):369-79. doi: 10.1016/0378-1119(84)90012-x.

Abstract

We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described.

MeSH terms

  • DNA Transposable Elements*
  • Escherichia coli / genetics*
  • Genetic Engineering / methods*
  • Genetic Vectors
  • Lac Operon*
  • Mutation*
  • Plasmids
  • beta-Galactosidase / genetics

Substances

  • DNA Transposable Elements
  • beta-Galactosidase