The pIC Plasmid and Phage Vectors With Versatile Cloning Sites for Recombinant Selection by Insertional Inactivation

Gene. 1984 Dec;32(3):481-5. doi: 10.1016/0378-1119(84)90022-2.

Abstract

The versatility of insertional inactivation of beta-galactosidase activity for subcloning and sequencing has been enhanced by combining a chemically synthesized oligonucleotide which specifies nine 6-bp-cutter restriction sites including BglII, XhoI, NruI, ClaI, SacI and EcoRV in various configurations with existing polylinkers to create a set of highly versatile cloning sites. These improved polylinkers have been inserted into plasmids (the pICs) for routine cloning of double-stranded DNA, and into chimeric phage/plasmids (the pICEMs) for biological production of single stranded DNA. The most versatile polylinker specifies 17 restriction sites in the beta-galactosidase alpha-complementing gene fragment. One of the new polylinkers was inserted into M13 DNA to produce a vector (M13mIC7) with nine cloning sites.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular / methods*
  • Coliphages / genetics
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Escherichia coli / genetics*
  • Galactosidases / genetics*
  • Genetic Vectors*
  • Plasmids
  • beta-Galactosidase / genetics*

Substances

  • DNA, Recombinant
  • DNA Restriction Enzymes
  • Galactosidases
  • beta-Galactosidase

Associated data

  • GENBANK/K02756
  • GENBANK/K02757
  • GENBANK/K02758
  • GENBANK/K02759
  • GENBANK/K02760