The sensitivity of opioid receptor types to regulation by sodium and GTP

Neuropeptides. 1984 Dec;5(1-3):137-40. doi: 10.1016/0143-4179(84)90046-5.

Abstract

The effects of Na+ and GTP on agonist binding to mu, delta, and kappa opioid binding sites in membranes from guinea pig cortex have been studied. All incubations were in a modified Krebs-Hepes buffer at 37 degrees; effects of Na+ were evaluated by replacement with an equal concentration of K+. mu binding sites appeared more sensitive than delta sites, while kappa sites were the least sensitive to both regulators. However, even at kappa sites, Na+ induced a significant reduction in agonist affinity and GTP caused a significant reduction in maximum binding.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cerebral Cortex / metabolism
  • Guanosine Triphosphate / pharmacology*
  • Guinea Pigs
  • In Vitro Techniques
  • Male
  • Receptors, Opioid / drug effects*
  • Receptors, Opioid, delta
  • Receptors, Opioid, kappa
  • Receptors, Opioid, mu
  • Sodium / pharmacology*

Substances

  • Receptors, Opioid
  • Receptors, Opioid, delta
  • Receptors, Opioid, kappa
  • Receptors, Opioid, mu
  • Guanosine Triphosphate
  • Sodium