Enhanced albumin production by malignantly transformed hepatocytes during in vitro exposure to dimethylsulfoxide

Biochim Biophys Acta. 1980 Nov 14;610(1):174-80. doi: 10.1016/0005-2787(80)90067-2.


The murine BW 1 and rat 32III 6/d tumor cell lines, derived from a spontaneous mouse hepatoma and a carcinogen-induced rat hepatocellular carcinoma, were used to investigate the effect of dimethylsulfoxide (DMSO) on liver cells in vitro. After a 4-day exposure to DMSO in final concentrations of 0.5 and 1.0%, BW 1 cell-associated albumin increased by 41.6 and 94.2%; extracellular albumin levels in these same cultures rose by 131.4 and 214.2%. Exposure of 32III 6/d cells to 2% DMSO produced increases in cell-associated and extra-cellular albumin concentrations of 67.8 and 188.7%, respectively. The lack of inducible gamma-glutamyl transpeptidase in BW 1 cells and its decrease in 32III 6/d cultures following DMSO treatment suggests that the DMSO-mediated enhancement of albumin production is not reflective of a random increase in the expression of cellular genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Albumins / biosynthesis*
  • Animals
  • Cell Line
  • Cell Transformation, Neoplastic / metabolism*
  • Clone Cells
  • Culture Media
  • Dimethyl Sulfoxide / pharmacology*
  • Fluorescent Antibody Technique
  • Liver / metabolism*
  • Liver Neoplasms, Experimental / metabolism*
  • Rats
  • gamma-Glutamyltransferase / metabolism


  • Albumins
  • Culture Media
  • gamma-Glutamyltransferase
  • Dimethyl Sulfoxide