Isolation and chemical characterization of somatostatin-28 from rat hypothalamus

Regul Pept. 1981 Nov;2(6):359-69. doi: 10.1016/0167-0115(81)90018-5.

Abstract

A peptide with somatostatin-like immuno- and bioactivity has been isolated from an aqueous extract of 96 800 rat hypothalamic by means of immunoaffinity chromatography, gel filtration and reverse-phase high-performance liquid chromatography (HPLC). Chemical characterization by amino acid analysis, tryptic peptide mapping and retention behavior in two HPLC system showed that the peptide was indistinguishable from somatostatin-28 previously characterized from several species. This evidence suggests that rat hypothalamic somatostatin-28 is identical in structure to porcine and ovine somatostatin-28.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Chromatography, Affinity
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Hypothalamus / analysis*
  • Molecular Weight
  • Protein Precursors / isolation & purification*
  • Rats
  • Somatostatin / isolation & purification*
  • Somatostatin-28

Substances

  • Amino Acids
  • Protein Precursors
  • Somatostatin
  • Somatostatin-28