The protochlorophyllide holochrome of barley (Hordeum vulgare L.). Phytochrome-induced decrease of translatable mRNA coding for the NADPH: protochlorophyllide oxidoreductase

Eur J Biochem. 1981 Nov;120(1):89-93. doi: 10.1111/j.1432-1033.1981.tb05673.x.


During the illumination of dark-grown barley plants light induces a rapid decrease of a translatable mRNA which codes for a polypeptide of Mr 44000. This component was identified as a precursor of the NADPH:protochlorophyllide oxidoreductase. The precursor has an Mr larger than the authentic protein by approximately 8000. The light-induced change in the level of translatable mRNA can be induced by a 15-s red-light pulse followed by 5 h of darkness. The red-light effect is reversed by a subsequent far-red-light treatment. It is concluded that the light-induced decline of translatable mRNA for the NADPH:protochlorophyllide oxidoreductase is controlled by phytochrome. The significance of this finding for present concepts of light-dependent control of chloroplast development and chlorophyll synthesis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Polyacrylamide Gel
  • Hordeum / enzymology
  • Molecular Weight
  • Oxidoreductases / genetics*
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Photic Stimulation
  • Phytochrome / metabolism*
  • Plant Proteins / metabolism*
  • Plants / enzymology*
  • Poly A / metabolism*
  • Protein Biosynthesis*
  • RNA, Messenger / metabolism*


  • Plant Proteins
  • RNA, Messenger
  • Phytochrome
  • Poly A
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • protochlorophyllide reductase