A stability-indicating assay for cefotaxime utilizing thin-layer chromatography with fluorescence detection

J Pharm Pharmacol. 1982 Jul;34(7):425-8. doi: 10.1111/j.2042-7158.1982.tb04749.x.


A t.l.c. method on silicagel plate with a fluorescence indicator has been carried out to increase the native fluorescence of cefotaxime and four of its degradation products. A simultaneous improved fluorimetric quantitation of the compounds in situ is proposed using a chromatogram spectrophotometer. The detectability (0.20 microgram) shows that the procedure is twenty times more sensitive than high-pressure liquid chromatography with u.v. detection; it allows the determination of traces level (1%) degradation products.

MeSH terms

  • Cefotaxime / analysis*
  • Chromatography, Thin Layer / methods
  • Drug Stability
  • Spectrometry, Fluorescence / methods


  • Cefotaxime