In situ determination of the molecular weight of hepatic gamma-glutamyl transferase and gamma-glutamyl hydrolase activities

Biochim Biophys Acta. 1982 Sep 22;707(1):164-6. doi: 10.1016/0167-4838(82)90410-1.

Abstract

The molecular weight of gamma-glutamyl transferase from normal rat liver and hepatoma tissue was determined by radiation-inactivation and found to be approx 100000 in each case. The molecular weight previously reported for the subunit containing the gamma-glutamyl binding site (22000) is considerably less than that of the holoenzyme, suggesting that in situ the large subunit is implicated in both transferase and hydrolase activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hydrolases* / radiation effects
  • Liver Neoplasms, Experimental / enzymology*
  • Microsomes / enzymology
  • Microsomes, Liver / enzymology*
  • Molecular Weight
  • Rats
  • Rats, Inbred Strains
  • gamma-Glutamyl Hydrolase
  • gamma-Glutamyltransferase* / radiation effects

Substances

  • gamma-Glutamyltransferase
  • Hydrolases
  • gamma-Glutamyl Hydrolase