RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase

J Virol. 1976 Sep;19(3):932-9. doi: 10.1128/JVI.19.3.932-939.1976.


Purified avian myeloblastosis virus (AMV) polymerase consisting of alpha,beta subunits has been shown to act processively in catalyzing DNA synthesis primed with 34S AMV RNA oligo(dT), poly(A)-poly(dT), and poly(I)-poly(dC). DNA transcripts prepared with 34S AMV RNA-oligo(dT)14 and AMV polymerase (alphabeta) have been shown to have a molecular weight of 1.05 X 10(6), or approximately one-third the size of the 34S RNA genome. Polymerase subunit alpha acts nonprocessively with the above templates.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Avian Leukosis Virus / enzymology*
  • Avian Myeloblastosis Virus / enzymology*
  • DNA, Viral / biosynthesis
  • Oligodeoxyribonucleotides / metabolism
  • Polynucleotides / metabolism
  • RNA, Viral / metabolism
  • RNA-Directed DNA Polymerase / metabolism*
  • Templates, Genetic
  • Transcription, Genetic


  • DNA, Viral
  • Oligodeoxyribonucleotides
  • Polynucleotides
  • RNA, Viral
  • RNA-Directed DNA Polymerase