Effect of isoprenaline on bethanechol-stimulated gastric antral motility in dogs with gastric fistula

Scand J Gastroenterol. 1982 Oct;17(7):939-44. doi: 10.3109/00365528209181118.


The purpose of the present study was to evaluate the effect of isoprenaline on gastric antral motility in conscious dogs with gastric fistula, using intraluminal strain-gauge transducers. Infusion of bethanechol increased the motility for both frequency and strength. Isoprenaline, a beta 1- and beta 2-agonist, was used alone and in conjunction with selective blockade of beta 1 and beta 2 receptors. The stimulated antral motility was dose-dependently inhibited by isoprenaline. The effect was significantly blocked by the beta 1 + beta 2-adrenoceptor blocker propranolol and by using in conjunction the beta 1-adrenoceptor blocker practolol and the beta 2-adrenoceptor blocker H 35/25. H 35/25 and particularly practolol reduced the effect of isoprenaline to some extent, but the reduction was not of statistical significance. This indicates that isoprenaline acts on antral motility through both beta 2 and beta 1 receptors. Dose-response experiments with five logarithmically increasing doses of bethanechol and one dose of isoprenaline showed inhibition of a non-competitive type.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Antagonists / pharmacology
  • Animals
  • Bethanechol Compounds / pharmacology*
  • Dogs
  • Dose-Response Relationship, Drug
  • Female
  • Gastrointestinal Motility / drug effects*
  • Isoproterenol / pharmacology*
  • Kinetics
  • Male
  • Pyloric Antrum / drug effects*


  • Adrenergic beta-Antagonists
  • Bethanechol Compounds
  • Isoproterenol