Prescreening for environmental teratogens using cultured mesenchymal cells from the human embryonic palate

Teratog Carcinog Mutagen. 1982;2(3-4):313-8. doi: 10.1002/1520-6866(1990)2:3/4<313::aid-tcm1770020312>3.0.co;2-c.

Abstract

Mesenchymal cells from the prefusion human embryonic palate have been established in culture and can be grown in either a serum-free hormone-supplemented medium or a serum-containing medium. The growth of these cells is quite rapid in culture and inhibited in a dose-dependent manner by most teratogens thus far tested, such as dexamethasone. These cells are highly sensitive to a variety of DNA synthetic and mitotic inhibitors. The responses of these cells are complementary to the ovarian tumor cell attachment assay of Braun et al [1, and in this volume]. When used in conjunction with the tumor cells, the overall reliability is greater than 90% with only one false-negative, allopurinol.

MeSH terms

  • Cells, Cultured
  • Dexamethasone / toxicity
  • Drug Evaluation, Preclinical / methods
  • Embryo, Mammalian / drug effects
  • Environmental Pollutants / toxicity*
  • Female
  • Humans
  • Palate / drug effects*
  • Palate / embryology
  • Pregnancy
  • Teratogens / toxicity*

Substances

  • Environmental Pollutants
  • Teratogens
  • Dexamethasone