Subcellular distribution and kinetic parameters of HS mouse liver aldehyde dehydrogenase

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;74(2):271-9. doi: 10.1016/0742-8413(83)90101-9.

Abstract

1. Aldehyde dehydrogenase subcellular distribution studies were performed in a heterogeneous stock (HS) of male and female mice (Mus musculus) with propionaldehyde (5 mM and 50 microM) and formaldehyde (1 mM) and NAD+ or NADP+. 2. The relative percents of distribution were: cytosolic 55-68%, mitochondrial 12-20%, microsomal 9-18% and lysosomal 3-15% for both propionaldehyde concentrations and NAD+. 3. Kinetic experiments using propionaldehyde and acetaldehyde with NAD+ revealed two separate enzymes, Enzyme I (low Km) and Enzyme II (high Km) in the cytosolic and mitochondrial fractions. 4. The kinetic data also indicated a spectrum of cytosolic low Km values that exhibited a bimodal distribution with one congruent to 40 microM and one congruent to 5 microM. 5. It was concluded that there was no significant difference in aldehyde-metabolizing capability between male and female HS mice, compared on a per gram of liver basis. The cytosolic low Km enzyme plays a major role in aldehyde oxidation at moderate to low aldehyde concentrations.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aldehyde Dehydrogenase
  • Aldehyde Oxidoreductases / metabolism*
  • Animals
  • Female
  • Formaldehyde / metabolism
  • Kinetics
  • Liver / enzymology*
  • Male
  • Mice
  • NAD / metabolism
  • Sex Factors
  • Subcellular Fractions / enzymology
  • Tissue Distribution

Substances

  • NAD
  • Formaldehyde
  • Aldehyde Oxidoreductases
  • Aldehyde Dehydrogenase