The stoichiometry of the Ca2+-pumping ATPase of erythrocytes

Cell Calcium. 1983 Apr;4(2):83-8. doi: 10.1016/0143-4160(83)90037-4.

Abstract

The stoichiometry of the erythrocyte Mg2+ dependent Ca2+-stimulated ATPase has been determined in a reconstituted system. Purified Ca2+ ATPase was incorporated into calcium impermeable liposomes and the ATP dependent calcium uptake was determined simultaneously with the hydrolysis of ATP. The results indicate that 1 gram atom of calcium is transported for each gram molecule of ATP hydrolysed, i.e., an ATP/Ca2+-stoichiometry of 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Ca(2+) Mg(2+)-ATPase
  • Calcium-Transporting ATPases / blood*
  • Calcium-Transporting ATPases / isolation & purification
  • Erythrocyte Membrane / enzymology*
  • Erythrocytes / enzymology*
  • Humans
  • Kinetics

Substances

  • Adenosine Triphosphate
  • Ca(2+) Mg(2+)-ATPase
  • Calcium-Transporting ATPases