Translocation of a lysosomal enzyme across the microsomal membrane requires signal recognition particle

Biochem Biophys Res Commun. 1983 Aug 30;115(1):275-80. doi: 10.1016/0006-291x(83)91000-8.

Abstract

Signal recognition particle (SRP), an 11S ribonucleoprotein (Walter and Blobel (1982) Nature 299, 691-698), is required for translocation of secretory proteins across microsomal membranes (Walter and Blobel (1980) Proc. Natl. Acad. Sci. USA 77, 7112-7116) and for asymmetric integration into microsomal membranes of a transmembrane protein (Anderson et al., (1982) J. Cell Biol. 93, 501-506). We demonstrate here that SRP is also required for translocation of the lysosomal protease cathepsin D across microsomal membranes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Cathepsin D
  • Cathepsins / genetics*
  • Dogs
  • Intracellular Membranes / metabolism
  • Lysosomes / enzymology*
  • Microsomes / enzymology*
  • Pancreas / enzymology*
  • Peptides / physiology*
  • Poly A / genetics
  • Protein Biosynthesis*
  • Protein Sorting Signals
  • RNA, Messenger / genetics
  • Spleen / enzymology*
  • Swine

Substances

  • Peptides
  • Protein Sorting Signals
  • RNA, Messenger
  • Poly A
  • Cathepsins
  • Cathepsin D