Supersensitivity of the GABA receptor in the frog spinal cord, as induced by kainic acid

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1983;76(2):231-6. doi: 10.1016/0742-8413(83)90070-1.

Abstract

In the isolated frog spinal cord perfused with kainic acid (KA, 5 X 10(-4) M) containing Ringer's solution, within 2 hr there were increases in the amplitude of the dorsal root depolarization, as induced by the GABA-agonists. KA perfusion produced increases in the specific binding of [3H]muscimol to crude synaptic membranes and incubation with KA for 3 hr did not increase [3H]muscimol binding. [3H]GABA was released from KA-treated spinal cord slices in the presence of high K+. KA-induced supersensitivity of the dorsal root to GABA may relate to direct actions on primary afferent terminals and not to denervation of GABAergic neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electrophysiology
  • In Vitro Techniques
  • Kainic Acid / pharmacology*
  • Muscimol / metabolism
  • Perfusion
  • Pyrrolidines / pharmacology*
  • Rana catesbeiana
  • Receptors, Cell Surface / physiology*
  • Receptors, GABA-A
  • Spinal Cord / physiology*
  • gamma-Aminobutyric Acid / metabolism*

Substances

  • Pyrrolidines
  • Receptors, Cell Surface
  • Receptors, GABA-A
  • Muscimol
  • gamma-Aminobutyric Acid
  • Kainic Acid