Characterization of Hantaan Virions, the Prototype Virus of Hemorrhagic Fever With Renal Syndrome

J Infect Dis. 1983 Dec;148(6):1005-12. doi: 10.1093/infdis/148.6.1005.

Abstract

Hantaan virus, strain 76-118, was propagated to high titer in a clone of Vero cells, and infectious virions were successfully concentrated and purified. Infectivity and virus antigenic activity were closely associated with a virus particle that exhibited a sedimentation rate indistinguishable from a representative member of the Bunyaviridae. Purified virions sedimented to a density of 1.16-1.17 in sucrose and 1.20-1.21 in cesium chloride. Detergent disruption of virions resulted in a nucleocapsid structure (density, 1.18 in sucrose and 1.25 in cesium chloride) and soluble protein antigens. Three separate nucleocapsids were resolved by rate-zonal centrifugation and contained a single but common polypeptide of 50,000 daltons. Electrophoresis of radiolabeled RNA extracted from purified virions yielded a profile of three RNA species with apparent molecular weights of 2.7, 1.2, and 0.6 X 10(6). These data support earlier electron microscopy reports which suggested that Hantaan virus has characteristics similar to some members of the virus family Bunyaviridae.

MeSH terms

  • Animals
  • Capsid / analysis
  • Cell Line
  • Centrifugation, Density Gradient
  • Chlorocebus aethiops
  • Hantavirus / analysis*
  • Hantavirus / isolation & purification
  • Hantavirus / physiology
  • Hemorrhagic Fever with Renal Syndrome / microbiology*
  • Humans
  • RNA Viruses / analysis*
  • RNA, Viral / analysis
  • Viral Proteins / analysis
  • Viral Structural Proteins
  • Virion / analysis
  • Virus Replication

Substances

  • RNA, Viral
  • Viral Proteins
  • Viral Structural Proteins