Simultaneous measurement of tyrosine, tryptophan and related monoamines for determination of neurotransmitter turnover in discrete rat brain regions by liquid chromatography with electrochemical detection

J Chromatogr. 1984 Jan 13;305(1):27-42. doi: 10.1016/s0378-4347(00)83311-3.


Concomitant measurement of monoamine neurotransmitter turnover in discrete rat brain areas with the use of radiolabeled amino acid precursors permits simultaneous evaluation of interacting transmitter systems. [3H]Tyrosine and [3H]tryptophan were administered via indwelling catheters to unrestrained rats. Content and specific activity of norepinephrine, dopamine, 5-hydroxytryptamine, and the metabolites dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindoleacetic acid in addition to tyrosine and tryptophan were quantified by liquid chromatography with electrochemical detection and liquid scintillation counting. The method employs a simple extraction procedure without prior cleanup for chromatography. Neurotransmitter turnover rates that incorporated tyrosine- or tryptophan-specific activities were found to be two to four times greater than those that did not include them.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biogenic Amines / analysis*
  • Brain Chemistry*
  • Chromatography, Liquid / methods
  • Electrochemistry
  • Male
  • Neurotransmitter Agents / analysis*
  • Rats
  • Serotonin / analysis
  • Tryptophan / analysis*
  • Tyrosine / analysis*


  • Biogenic Amines
  • Neurotransmitter Agents
  • Serotonin
  • Tyrosine
  • Tryptophan