Mol Cell Biochem. 1984;58(1-2):9-35. doi: 10.1007/BF00240602.


This paper is intended as a background to the topic of transglutaminases, while focusing on current ideas regarding the biological roles of these enzymes. Specifically, the following topics are discussed: geometry of forming gamma-glutamyl-epsilon-lysine cross-linked structures; energetic considerations; the gamma-glutamyl-epsilon-lysine cross-link; amine incorporation assays; artefactual incorporation of amines in cells and tissue homogenates; synthetic substrate systems; regulation of transglutaminase activities; strategies for probing transglutaminase-mediated events in biological systems; the blood clotting paradigm; transglutaminase and cell aging: the Ca2+-enriched human erythrocyte; transglutaminase and cell activation: the thrombin-stimulated human platelet and the fertilized sea urchin egg.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Acyltransferases / antagonists & inhibitors
  • Acyltransferases / physiology*
  • Amines / metabolism
  • Animals
  • Blood Coagulation
  • Blood Platelets / physiology
  • Calcium / blood
  • Cell Survival
  • Enzyme Activation
  • Erythrocyte Aging
  • Female
  • Fertilization
  • Glutamates
  • Kinetics
  • Lysine
  • Ovum / physiology
  • Substrate Specificity
  • Thermodynamics
  • Transglutaminases


  • Amines
  • Glutamates
  • Acyltransferases
  • Transglutaminases
  • Lysine
  • Calcium