The human carcinoma cell line A431 possesses large numbers of functional beta-adrenergic receptors

FEBS Lett. 1984 Apr 24;169(2):151-5. doi: 10.1016/0014-5793(84)80308-7.

Abstract

The existence of beta-adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1-[3H]dihydroalprenolol and the hydrophilic antagonist [3H]CGP-12,177 as beta-adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the beta 2-subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human beta-adrenergic receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Antagonists / metabolism
  • Binding, Competitive
  • Carcinoma, Squamous Cell / metabolism*
  • Cell Line
  • Dihydroalprenolol / metabolism
  • Humans
  • Isoproterenol / metabolism
  • Propanolamines / metabolism
  • Propranolol / metabolism
  • Receptors, Adrenergic, beta / metabolism*
  • Stereoisomerism

Substances

  • Adrenergic beta-Antagonists
  • Propanolamines
  • Receptors, Adrenergic, beta
  • Dihydroalprenolol
  • Propranolol
  • Isoproterenol
  • CGP 12177