Immunocytochemical localization of GABA neurons and dopamine neurons in the rat main and accessory olfactory bulbs

Neurosci Lett. 1984 Jun 29;47(3):221-6. doi: 10.1016/0304-3940(84)90517-2.

Abstract

Immunocytochemical localization of GABA neurons and dopamine neurons in the rat olfactory bulb was obtained with sheep antiserum to glutamate decarboxylase (GAD) and rabbit antiserum to tyrosine hydroxylase (TH). GAD-positive neurons include periglomerular cells, granule cells, superficial and deep short axon cells. TH-positive neurons represent periglomerular cells. Two-color immunocytochemistry shows that GABA and dopamine periglomerular cells are separate populations. The accessory olfactory bulb has rare dopamine cells and few superficial short axon cells. Radial gradients of GAD-immunostaining are evident in the main but not in the accessory olfactory bulb.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Central Nervous System / enzymology*
  • Dopamine / metabolism*
  • Glutamate Decarboxylase / metabolism
  • Immunoenzyme Techniques
  • Microscopy, Electron
  • Nerve Net / enzymology
  • Neurons / classification
  • Neurons / enzymology
  • Olfactory Bulb / enzymology*
  • Olfactory Nerve / enzymology
  • Olfactory Pathways / enzymology*
  • Rats
  • Rats, Inbred Strains
  • Synapses / enzymology
  • Synaptic Transmission*
  • Tyrosine 3-Monooxygenase / metabolism
  • gamma-Aminobutyric Acid / metabolism*

Substances

  • gamma-Aminobutyric Acid
  • Tyrosine 3-Monooxygenase
  • Glutamate Decarboxylase
  • Dopamine