On the binding of labelled saxitoxin to the squid giant axon

Proc R Soc Lond B Biol Sci. 1984 Aug 22;222(1227):147-53. doi: 10.1098/rspb.1984.0055.

Abstract

Measurement of the maximum saturable binding capacity for 3H-labelled saxitoxin in well cleaned giant axons from Loligo forbesi gave a value of 290 +/- 65 (s.e.m.) binding sites per square micrometre of membrane. In samples of the fin nerve the total saturable uptake was 12.4 +/- 2.4 fmol mg-1 wet mass, corresponding to 94 sites per square micrometre for fibres whose mean diameter after fixation and embedding was estimated as 16 micron.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axons / metabolism*
  • Binding Sites
  • Decapodiformes
  • Saxitoxin / metabolism*

Substances

  • Saxitoxin