Isolated, mononucleated, striated muscle can undergo pluripotent transdifferentiation and form a complex regenerate

Cell. 1984 Oct;38(3):801-9. doi: 10.1016/0092-8674(84)90275-7.


Isolated, mononucleated, cross-striated muscle of a medusa can be activated by collagenase treatment to transdifferentiate completely to various new cell types and to regenerate autonomously the sexual (without gametes) and feeding organ of the animal. Under these circumstances all isolated muscle fragments produce smooth muscle cells and a glandular cell type (y-cells). When culture conditions are appropriate, endoderm is also formed, followed by regeneration of a complex organ of seven or eight new non-muscle cell types, including nematocytes, digestive, secretory, gland, interstitial, and presumably nerve cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Cnidaria
  • Cytoskeleton / ultrastructure
  • DNA Replication
  • Microbial Collagenase
  • Microscopy, Electron
  • Muscles / cytology*
  • Muscles / ultrastructure
  • Regeneration


  • Microbial Collagenase