Reduced maximum capacity of glycolysis in brown adipose tissue of genetically obese, diabetic (db/db) mice and its restoration following treatment with a thermogenic beta-adrenoceptor agonist

FEBS Lett. 1984 Oct 15;176(1):16-20. doi: 10.1016/0014-5793(84)80903-5.


The maximal activities of the key glycolytic enzymes hexokinase and 6-phosphofructokinase, were reduced in brown adipose tissue in db/db mice compared to their lean littermates. Treatment of db/db mice with the thermogenic beta-adrenoceptor agonist, BRL 26830, restored normoglycaemia. The only significant increase in activity of hexokinase and 6-phosphofructokinase in the BRL 26830-treated db/db mice occurred in brown adipose tissue where the total tissue activity increased 10- and 11-fold respectively. These changes together with increased 2-deoxyglucose uptake in vivo suggest that brown adipose tissue can play a quantitatively important role in the removal of glucose from the blood.

MeSH terms

  • Adipose Tissue, Brown / enzymology*
  • Adrenergic beta-Agonists / pharmacology
  • Animals
  • Deoxyglucose / metabolism
  • Diabetes Mellitus, Experimental / metabolism*
  • Ethanolamines / pharmacology*
  • Female
  • Glycolysis / drug effects*
  • Hexokinase / metabolism
  • Ketoglutarate Dehydrogenase Complex / metabolism
  • Liver / enzymology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Obese
  • Muscles / enzymology
  • Obesity / metabolism*
  • Phosphofructokinase-1 / metabolism


  • Adrenergic beta-Agonists
  • Ethanolamines
  • BRL 26830A
  • Deoxyglucose
  • Ketoglutarate Dehydrogenase Complex
  • Hexokinase
  • Phosphofructokinase-1