Evidence for two somatostatin-14 receptor types in rat brain cortex

Neurosci Lett. 1984 Aug 31;49(3):259-63. doi: 10.1016/0304-3940(84)90299-4.


The binding characteristics of a stable somatostatin analogue, SMS 201-995, have been evaluated in a somatostatin receptor binding assay using [125I-Tyr11]somatostatin, and cortical, pituitary or pancreatic membranes. High affinity binding sites for somatostatin-14 and for SMS 201-995 were identified in rat cortex (Kd = 0.60 nM) as well as in pituitary (Kd = 0.74 nM) and pancreatic beta-cells (Kd = 0.18 nM). In the cortex, in contrary to the two other tissues, SMS 201-995 only labels a part (approximately 75%) of the receptors recognized by somatostatin-14. This suggests the presence in the cortex of more than one population of somatostatin receptors. In conclusion, SMS 201-995 appears as a valuable tool for differentiating the two somatostatin receptor types.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Binding, Competitive
  • Cerebral Cortex / analysis*
  • Cerebral Cortex / metabolism
  • Islets of Langerhans / metabolism
  • Octreotide
  • Pituitary Gland, Anterior / metabolism
  • Radioligand Assay
  • Rats
  • Receptors, Cell Surface / analysis*
  • Receptors, Somatostatin
  • Somatostatin / analogs & derivatives
  • Somatostatin / metabolism


  • Receptors, Cell Surface
  • Receptors, Somatostatin
  • Somatostatin
  • somatostatin, iodo-Tyr(1)-
  • Octreotide