Thiazolidine-2-carboxylate derivatives formed from glyoxylate and L-cysteine or L-cysteinylglycine as possible physiological substrates for D-aspartate oxidase

Biochem Biophys Res Commun. 1984 Dec 28;125(3):1039-45. doi: 10.1016/0006-291x(84)91388-3.

Abstract

Adducts of glyoxylate with L-cysteine or L-cysteinylglycine were found to be excellent substrates at low concentrations for beef kidney D-aspartate oxidase. Evidence is presented that cis-thiazolidine-2,4-dicarboxylate and its glycine amide are the actual substrates, and that both are converted in the enzymic reaction to 4-substituted thiazoline-2-carboxylates. The results imply that these thiazolidine derivatives are the likely physiological reactants for mammalian D-aspartate oxidase.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / metabolism*
  • Animals
  • Aspartic Acid / metabolism
  • Cattle
  • Cysteine / metabolism
  • D-Aspartate Oxidase
  • Dipeptides / metabolism
  • Glutamates / metabolism
  • Glutamic Acid
  • Glyoxylates / metabolism
  • Hydrogen-Ion Concentration
  • Proline / analogs & derivatives*
  • Proline / metabolism
  • Substrate Specificity
  • Thiazolidines

Substances

  • Dipeptides
  • Glutamates
  • Glyoxylates
  • Thiazolidines
  • thiazolidine-2-carboxylic acid
  • Aspartic Acid
  • cysteinylglycine
  • Glutamic Acid
  • Proline
  • Amino Acid Oxidoreductases
  • D-Aspartate Oxidase
  • glyoxylic acid
  • Cysteine