Legionella pneumophila: identification in tissue sections by a new immunoenzymatic procedure

Arch Pathol Lab Med. 1980 Jun;104(6):283-6.

Abstract

A modified glucose oxidase immunoenzyme technique was shown to be highly sensitive and specific for detection of serogroup 1 Legionella pneumophila in 4% formaldehyde solution-fixed, paraffin-embedded tissue sections. There was complete concordance between infection with L pneumophila and detection of the organisms in tissue sections by glucose oxidase immunoenzyme staining. The L pneumophila organisms stained blue-black and were found within phagocytic cells as well as in the extracellular space. A cloud of blue-black pigment, probably representing diffusable antigen, was present in the extracellular spaces in the area of L pneumophila localization. No false-positive or false-negative reactions were found. This technique requires no specialized equipment, may be applicable to retrospective diagnostic problems, and can be adapted to routine diagnostic practice.

MeSH terms

  • Animals
  • Bacteria / isolation & purification*
  • Glucose Oxidase*
  • Guinea Pigs
  • Humans
  • Immune Sera / immunology
  • Immunoenzyme Techniques*
  • Legionnaires' Disease / immunology
  • Legionnaires' Disease / microbiology*
  • Lung / microbiology
  • Rabbits / immunology
  • Staining and Labeling

Substances

  • Immune Sera
  • Glucose Oxidase