Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red S

Teratology. 1980 Dec;22(3):299-301. doi: 10.1002/tera.1420220306.


The procedure described by Inouye ('76) for the staining of full-term mouse fetal skeletons has been adapted for use with mouse embryos and fetuses of days 14-18 of gestation. The main adaptations for younger specimens involve a longer time in acetone, in lieu of skinning, and omission of the aqueous KOH step. These adaptations require more time but result in consistently good staining of intact specimens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcian Blue
  • Animals
  • Anthraquinones
  • Bone and Bones / anatomy & histology*
  • Cartilage / anatomy & histology*
  • Female
  • Fetus / anatomy & histology*
  • Gestational Age
  • Histological Techniques*
  • Mice
  • Pregnancy
  • Staining and Labeling


  • Anthraquinones
  • Alizarin Red S
  • Alcian Blue