Two rates of fast axonal transport of [3H]glycoprotein in an identified invertebrate neuron

Brain Res. 1981 Dec 21;229(2):445-55. doi: 10.1016/0006-8993(81)91006-4.

Abstract

Recently, the kinetics of fast axonal transport of a single type of organelle, the serotonergic storage vesicle, was described in an identified axon of Aplysia using a pulsing technique combined with intracellular injection of [3H]serotonin. Here we extended the single axon studies by analyzing the movement of pulses of [3H]glycoprotein, following injection into the giant Aplysia cell, R2, of the amino sugar [3H]N-acetylgalactosamine. This glycoprotein precursor has been shown to label several organelles in this neuron. [3H]Glycoprotein is found to move in the axon of R2 at 2 rates of fast transport, 174 and 105 mm per day at room temperature. We suggest that the 2 rates reflect movements of 2 different types of organelle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylgalactosamine / metabolism
  • Animals
  • Aplysia
  • Axonal Transport*
  • Endoplasmic Reticulum / metabolism
  • Ganglia / metabolism
  • Glycoproteins / metabolism*
  • Kinetics
  • Molecular Weight
  • Neurons / metabolism
  • Serotonin / metabolism
  • Synaptic Vesicles / metabolism

Substances

  • Glycoproteins
  • Serotonin
  • Acetylgalactosamine