Two-dimensional rhodopsin crystals from disk membranes of frog retinal rod outer segments

Proc Natl Acad Sci U S A. 1982 Feb;79(4):1116-20. doi: 10.1073/pnas.79.4.1116.

Abstract

Two-dimensional crystals of rhodopsin have been prepared from purified frog disk membranes by using the detergent Tween 80. The space group of the orthorhombic crystals is p22121; the unit cell dimensions are 47 X 151 A. Projection maps of negatively stained preparations have been calculated to a resolution of approximately 22 A. The rhodopsin molecules are associated as dimers that appear to be slightly sigmoidal and are 20-25 A in width and 70-80 A in length.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Computers
  • Crystallization
  • Microscopy, Electron
  • Photoreceptor Cells / analysis*
  • Rana catesbeiana
  • Retinal Pigments* / analysis
  • Rhodopsin* / analysis
  • Rod Cell Outer Segment / analysis*
  • Staining and Labeling

Substances

  • Retinal Pigments
  • Rhodopsin