Properties of dispersed, highly repeated DNA within and near the hamster CAD gene

Mol Cell Biol. 1982 Mar;2(3):302-7. doi: 10.1128/mcb.2.3.302.

Abstract

Dispersed, highly repeated DNA sequences were found within and near the Syrian hamster gene coding for the multifunctional protein CAD. Most of the repeated sequences were homologous to each other and had similar properties. They hybridized to many cytoplasmic polyadenylated RNAs and to 7S and 4.5S cytoplasmic non-polyadenylated RNAs. Cloned DNA fragments containing repeated sequences were transcribed in vitro by RNA polymerase III. The repeated sequences from Syrian hamsters share many properties with the Alu family of repetitive DNA from humans. The hamster sequences were homologous to total repetitive human DNA but only very weakly homologous to two cloned members of the human Alu family.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Aspartate Carbamoyltransferase / genetics
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics
  • Cells, Cultured
  • Cricetinae
  • DNA, Recombinant
  • Dihydroorotase / genetics
  • Humans
  • Kidney
  • Mesocricetus
  • Multienzyme Complexes / genetics*
  • Nucleic Acid Hybridization
  • RNA / analysis
  • RNA Polymerase III / metabolism
  • Repetitive Sequences, Nucleic Acid*
  • Transcription, Genetic

Substances

  • DNA, Recombinant
  • Multienzyme Complexes
  • RNA
  • Aspartate Carbamoyltransferase
  • RNA Polymerase III
  • Dihydroorotase
  • Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)