Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate

Cell. 1982 Jun;29(2):403-15. doi: 10.1016/0092-8674(82)90157-x.


Duck hepatitis B virus, a DNA virus closely related to human hepatitis B virus, was studied in infected duck liver. Subviral particles resembling the viral nucleocapsid cores were isolated from persistently infected liver and shown to have a DNA polymerase activity that utilizes an endogenous template and synthesizes both plus- and minus-strand viral DNA. Synthesis of the viral minus-strand DNA utilized an RNA template that was degraded as it was copied. Viral plus-strand synthesis occurred on the completed minus-strand DNA. A pathway for the replication of the DNA genome of hepatitis B-like viruses by reverse transcription of an RNA intermediate is proposed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA, Viral / biosynthesis
  • DNA-Directed DNA Polymerase / physiology
  • Dactinomycin / pharmacology
  • Deoxyribonucleases / pharmacology
  • Ducks
  • Genes, Viral
  • Hepatitis B virus / physiology*
  • Liver / metabolism
  • RNA, Viral / physiology
  • RNA-Directed DNA Polymerase / metabolism*
  • Ribonucleases / pharmacology
  • Virus Replication*


  • DNA, Viral
  • RNA, Viral
  • Dactinomycin
  • RNA-Directed DNA Polymerase
  • DNA-Directed DNA Polymerase
  • Deoxyribonucleases
  • Ribonucleases