A Simplified Timm Stain Procedure Compatible With Formaldehyde Fixation and Routine Paraffin Embedding of Rat Brain

Brain Res Bull. 1982 Jun;8(6):771-4. doi: 10.1016/0361-9230(82)90104-6.


A simplified sulphide/silver technique for visualizing transitional metals in rat brain is described. It consists of: (1) sulphide perfusion before formaldehyde perfusion, (2) routine paraffin embedding and sectioning, and (3) silver staining with physical developer. This method is compatible with formaldehyde fixation, eliminates the need for post-fixation in Carnoy's solution before embedding and does not require low paraffin temperatures during embedding. Once embedded, brains can be stored for months before sectioning without loss of stain quality.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Brain / cytology*
  • Formaldehyde
  • Hippocampus / cytology
  • Histological Techniques
  • Male
  • Paraffin
  • Perfusion
  • Rats
  • Staining and Labeling
  • Sulfides
  • Zinc / analysis


  • Sulfides
  • Formaldehyde
  • Paraffin
  • Zinc