Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain

Nucleic Acids Res. 1982 Oct 11;10(19):5925-34. doi: 10.1093/nar/10.19.5925.

Abstract

We report the cloning of five overlapping cDNAs bearing sequences specific for the human pro alpha 1(I) collagen chain. Poly-A RNA enriched for collagen sequences was purified from normal human fibroblasts and used as template to synthesize double stranded cDNA. The cDNA was inserted into the Eco RI site of pBR 322 by blunt-ending and dG:dC tailing. The clones were screened by colony hybridization using the original RNA population and the resulting five positive clones subjected to restriction endonuclease mapping analysis and DNA sequencing. These overlapping clones cover from residue 247 in the alpha chain to part of the 3' end untranslated region of the pro alpha 1(I) mRNA for a total of 3400 nucleotides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • DNA / analysis*
  • DNA Restriction Enzymes
  • Genes*
  • Humans
  • Nucleic Acid Hybridization
  • Poly A / genetics
  • Procollagen / genetics*
  • RNA / genetics
  • RNA, Messenger

Substances

  • Procollagen
  • RNA, Messenger
  • Poly A
  • RNA
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J00110
  • GENBANK/J00111
  • GENBANK/J00112
  • GENBANK/J00113