Mutations in the rpIJ leader of Escherichia coli that abolish feedback regulation

Cell. 1983 Feb;32(2):361-9. doi: 10.1016/0092-8674(83)90455-5.

Abstract

We have isolated mutants that fail to exhibit biosynthetic feedback regulation of a rpIJ-lacZ fusion. Analysis of these mutants and of others that were isolated earlier indicates that crucial sequences for both translational feedback regulation and efficient translation lie closely intermingled in the central region of the rpIJ mRNA leader 70-195 bases upstream from the translation start of rpIJ. We suggest that our point mutations define a region of the rpIJ leader mRNA to which L10 binds in effecting autogenous translational regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Base Sequence
  • DNA, Recombinant
  • Escherichia coli / genetics*
  • Gene Expression Regulation*
  • Mutation
  • RNA, Bacterial / genetics*
  • RNA, Bacterial / metabolism
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Ribosomal Protein L10
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / metabolism

Substances

  • Bacterial Proteins
  • DNA, Recombinant
  • RNA, Bacterial
  • RNA, Messenger
  • Ribosomal Proteins