Hypomethylation of ras oncogenes in primary human cancers

Biochem Biophys Res Commun. 1983 Feb 28;111(1):47-54. doi: 10.1016/s0006-291x(83)80115-6.


We have examined the methylation status of two cellular oncogenes, c-Ha-ras and c-Ki-ras, in primary human carcinomas and the adjacent analogous normal tissues from which the tumors derived. The c-Ha-ras gene was hypomethylated in six of eight carcinomas, including five colonic adenocarcinomas and one small cell lung carcinoma, when compared to adjacent normal tissues. The c-Ki-ras gene was hypomethylated to a lesser extent in two colonic adenocarcinomas. This is the first demonstration of alterations in methylation of cellular oncogenes in human cancer.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / genetics
  • Carcinoma, Small Cell / genetics
  • Colonic Neoplasms / genetics
  • DNA Restriction Enzymes / metabolism
  • DNA, Neoplasm / analysis
  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific*
  • Humans
  • Lung Neoplasms / genetics
  • Methylation
  • Neoplasms / genetics*
  • Nucleic Acid Hybridization
  • Oncogenes*
  • Polymorphism, Genetic


  • DNA, Neoplasm
  • DNA Restriction Enzymes
  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific
  • GCGC-specific type II deoxyribonucleases