Transganglionic demonstration of central sensory projections from skin and muscle with HRP-lectin conjugates

Neurosci Lett. 1980 Apr;17(1-2):1-6. doi: 10.1016/0304-3940(80)90051-8.


Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axonal Transport
  • Biological Transport
  • Female
  • Histocytochemistry / methods
  • Horseradish Peroxidase*
  • Lectins*
  • Muscles / innervation*
  • Neuroanatomy / methods*
  • Neurons, Afferent
  • Peroxidases*
  • Rats
  • Skin / innervation*
  • Spinal Cord / anatomy & histology*
  • Wheat Germ Agglutinins


  • Lectins
  • Wheat Germ Agglutinins
  • Horseradish Peroxidase
  • Peroxidases