Location and distribution of the light chains in clathrin trimers

Proc Natl Acad Sci U S A. 1983 May;80(9):2481-5. doi: 10.1073/pnas.80.9.2481.

Abstract

Mouse monoclonal antibodies have been made that are specific for one of the light chains (LC-A) of calf brain clathrin. The determinant they recognize has been mapped by immunoelectron microscopy and shown to lie near the center of a clathrin trimer. Quantitative immunoprecipitation experiments with one of the monoclonal antibodies suggests that, despite the overall 1:2 stoichiometry, the light chain composition of an individual clathrin trimer is nearly random.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Brain Chemistry
  • Clathrin
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes / analysis
  • Female
  • Macromolecular Substances
  • Membrane Proteins / analysis*
  • Mice
  • Mice, Inbred BALB C
  • Microscopy, Electron

Substances

  • Antibodies, Monoclonal
  • Clathrin
  • Epitopes
  • Macromolecular Substances
  • Membrane Proteins